Research cluster: PathControl / Subproject |
| Subproject 2: Effect of Bacillus amyloliquefaciens on the rhizoplane microbial community and on gene expression of the plant pathogen Rhizoctonia solani |
| Project time: 01.01.10-30.06.13 |
| Project leader: |
| Prof. Dr. Alfred Pühler, Universität Bielefeld |
| Dr. Andreas Schlüter, Universität Bielefeld |
| Analysis of the interaction between the biocontrol strain Bacillus amyloliquefaciens FZB42 and the phytopathogenic fungus Rhizoctonia solani in the root zone of the model plant Lactuca sativa (lettuce) is in the focus of this sub-project. To enable an analysis at the molecular level, it is necessary to determine the genome sequence of the phytopathogen Rhizoctonia solani (anastomosis group AG1-IB). An oligonucleotide microarray including R. solani pathogenicity and virulence determinants as well as genes that play a role for fungal growth and infection will be applied to analyse the transcriptional profile of R. solani in dependence of the biocontrol strain. |
 |
| Moreover, the influence of R. solani on the transcription of B. amyloliquefaciens genes will also be addressed. For this approach an approved B. amyloliquefaciens microarray is available. It is expected that this analysis will uncover genetic determinants that are important for the biocontrol properties of the strain FZB42 against the fungus R. solani. On the other hand, analysis of the R. solani transcriptional profile in dependence of the antagonist B. amyloliquefaciens FZB42 should provide insights into R. solani pathogenicity features and functions that play a role in virulence and infection. Furthermore, the effect of the biocontrol strain FZB42 on the composition of the microbial community on the L. sativa rhizoplane will be analysed by means of a metagenome approach. |
 |
Funded by
New Brochure: Facetten der Genomforschung
Wissenschaftler entschlüsseln die Baupläne des Lebens (german; pdf; 12,3 Mb)
Wissenschaftler entschlüsseln die Baupläne des Lebens (german; pdf; 12,3 Mb)
