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Research project: Autotrophe Produktion
 
Autotrophic production of fine chemicals and specifically labeled biocompounds in Ralstonia eutropha
Project time: 01.09.09-31.03.13
Cluster coordinator: Prof. Dr. Bärbel Friedrich, Humboldt-Universität zu Berlin, Berlin
Project partners: (alphabetical order)
   Prof. Dr. Bärbel Friedrich, Humboldt-Universität zu Berlin
   Prof. Dr. Michael Hecker, Ernst-Moritz-Arndt-Universität Greifswald
   Dr. Hermann Heumann, Silantes GmbH
   Prof. Dr. Rudibert King, Technische Universität Berlin
   Prof. Dr. Alexander Steinbüchel, Westf. Wilhelms-Universität Münster

      Overview subprojects


Industrial partners:
   Silantes GmbH, München

The collaborative research project "Autotrophic production" focuses on the improvement and establishment of new processes for an economically attractive production of stable isotope (SI)-labeled biomolecules and fine chemicals. SI-labeled biomolecules are used in multidimensional NMR and quantitative mass spectrometry.
The enrichment of stable isotopes by lithoautotrophic fermentation of autotrophic bacteria is efficient and economic. The facultative lithoautotrophic proteobacteria R. eutropha is already used for the industrial production of SI-biomolecules. The research project will include metabolic engeneering of the strain for the production of polymers and proteins under autotrophic growth conditions and the development of new expression systems for improved overproduction of proteins under these conditions. Proteome and transcriptome analysis of the organism will serve as a basis for successfull optimization of the strain and indexing intra- and extracellular metabolites will enable to identify new substances that are useful for SI-labeling. The project will integrate process engineering and mathematical modeling of the production process.

References:

Peplinski K, Ehrenreich A, Döring C, Bömeke M, Reinecke F, Hutmacher C, Steinbüchel A. (2010) Genome-wide transcriptome analyses of the 'Knallgas' bacterium Ralstonia eutropha H16 with regard to polyhydroxyalkanoate metabolism. Microbiology 156:2136-52. http://www.ncbi.nlm.nih.gov/pubmed/20395272
Schwartz E, Voigt B, Zühlke D, Pohlmann A, Lenz O, Albrecht D, Schwarze A, Kohlmann Y, Krause C, Hecker M, Friedrich B. (2009) A proteomic view of the facultatively chemolithoautotrophic lifestyle of Ralstonia eutropha H16. Proteomics. 9(22):5132-42. http://www.ncbi.nlm.nih.gov/pubmed/19798673
Pohlmann A, Fricke WF, Reinecke F, Kusian B, Liesegang H, Cramm R, Eitinger T, Ewering C, Pötter M, Schwartz E, Strittmatter A, Voss I, Gottschalk G, Steinbüchel A, Friedrich B, Bowien B. (2006) Genome sequence of the bioplastic-producing "Knallgas" bacterium Ralstonia eutropha H16. Nat Biotechnol. 24(10):1257-62. http://www.ncbi.nlm.nih.gov/pubmed/16964242
Fleige C, Kroll J, Steinbüchel A (2011) Establishment of an alternative phosphoketolase-dependent pathway for fructose catabolism in Ralstonia eutropha H16. Appl Microbiol Biotechnol 91:769-776.