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Research project: PathControl
 
A molecular analysis of the genetic determinants affecting ability to grow of the plant growth promoting bacterium Bacillus amyloliquefaciens within plant rhizosphere (lettuce) in presence of the world-wide distributed phytopathogenic fungus Rhizoctonia solani
Project time: 01.01.10-30.06.13
Cluster coordinator: Prof. Dr. Rainer Borriss, ABiTEP GmbH, Berlin
Project partners: (alphabetical order)
   Prof. Dr. Rainer Borriss, ABiTEP GmbH
   Prof. Dr. Anton Hartmann, Helmholtz Zentrum München
   Prof. Dr. Alfred Pühler, Universität Bielefeld
   Dr. Andreas Schlüter, Universität Bielefeld
   PD Dr. Philippe Schmitt-Kopplin, Helmholtz Zentrum München

      Overview subprojects


Industrial partners:
   ABiTEP GmbH, Berlin

A molecular analysis of the genetic determinants affecting ability to grow of the plant growth promoting bacterium Bacillus amyloliquefaciens [1,2] within plant rhizosphere (lettuce) in presence of the world-wide distributed phytopathogenic fungus Rhizoctonia solani will be performed. Antagonistic interactions between the hostile fungus and the benefical bacterium are of special interest.
Expression of the “root colonizing” and “biocontrol” genes on the level of transcription (RNA/DNA-Microarray) and translation (“proteomics”) are investigated. For developing an efficient biocontrol agent based on B. amyloliquefaciens the molecular reason(s) for the beneficial effect of FZB42 have to be elucidated further: (i) production of antifungal secondary metabolites within plant rhizosphere, (ii) direct growth competition during colonizing of plant roots, (iii) Enhancement of induced systemic resistance (ISR) directed against the phytopathogenic fungus by the beneficial bacterium. Establishing a draft genome sequence of Rhizoctonia and designing appropriate microchips bearing oligonucleotides derived from the relevant genes should enable global analysis of the fungal gene expression under conditions of plant rhizosphere and in presence and abscence of the beneficial bacterium. Moreover, possible effects of the antagonistic microorganisms on the microbial soil population in vicinity of plant roots were followed by metagenomic approaches.

References:

1. Chen et al. (2007) Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42. Nature Biotechnol. 25(9):1007-14.
2. Borriss et al. (2011) Relationship of Bacillus amyloliquefaciens clades associated with strains DSM7T and FZB42: a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on their discriminating complete genome sequences. Int J Syst Evol Microbiol. 61(8):1786-1801.